Whole-genome sequencing in an autism multiplex family

Whole-genome sequencing in an autism multiplex family

BACKGROUND Autism spectrum disorders (ASDs) represent a group of childhood neurodevelopmental disorders that affect 1 in 88 children in the US. Previous exome sequencing studies on family trios have implicated a role for rare, de-novo mutations in the pathogenesis of autism. METHODS To examine the utility of whole-genome sequencing to identify inherited disease candidate variants and genes, w...

Whole Genome Sequencing: An Efficient Approach in Food Safety Management System

An Algorithm for Whole Genome Shotgun Sequencing

The push to sequence the entire human genome is gearing up [1]. Recently there has been disagreement within the genome community as to the best approach for sequencing the human genome. While many scientists believe that clone mapping is the best solution, others argue that whole genome shotgun sequencing is a cheaper and faster alternative. Arguments for [2] and against [3] whole genome shotgu...

whole genome sequencing: an efficient approach in food safety management system

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Whole-Genome Bisulfite Sequencing

Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...